Journal: PLOS Pathogens

Article Title: Nucleus softens during herpesvirus infection

doi: 10.1371/journal.ppat.1013873

Figure Lengend Snippet: (A) Nuclear lamina labeled by lamin A chromobody, skeletonization of the lamina, and discretized lamina points showing regions of positive (green) and negative curvature (red) in infected Vero cells at 8 hpi. (B) The fraction of negative curvature points for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (C) A zoom-in of the nuclear lamina, showing its motion and maximum intensity projection of the skeleton during 24.8 s. Scale bars, 5 µm. (D) Nuclear lamina mobility, measured as the area covered by the skeleton during one minute for noninfected cells and cells imaged 8 and 12 hpi (n = 19, 22, and 17, respectively). (E) Nuclear envelope (NE) tension analysis by fluorescence lifetime imaging microscopy (FLIM) of noninfected and HSV-1 17 + wild-type virus-infected Vero cells at 12 hpi using fluorescence lifetime-reporting membrane tension probe ER Flipper-TR. A fast FLIM image presented in a pseudocolor scale representing average photon arrival times in each pixel, ranging from 2.0 to 2.6 ns (calibration bar). Representative cells with average lifetime distribution, segmented NE (red) around the Hoechst signal (grey), and average lifetime at the NE region are shown. Scale bar, 10 µm. (F) The longer lifetime component of the ER Flipper probe, responsive to the membrane tension, in noninfected and infected cells at 8 and 12 hpi (n = 51, 58, and 59, respectively). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05) or **** (p < 0.0001). Nonsignificant differences (p ≥ 0.05) are not labeled.

Article Snippet: Nuclear membrane tension was analyzed by FLIM using fluorescent membrane tension probe ER Flipper-TR (Spirochrome) in noninfected and HSV-1 17 + wt infected live Vero cells at 8 and 12 hpi.

Techniques: Labeling, Infection, Fluorescence, Imaging, Microscopy, Virus, Membrane, Standard Deviation

Journal: Journal of Nanobiotechnology

Article Title: Engineered endoplasmic reticulum-targeting nanodrugs with Piezo1 inhibition and promotion of cell uptake for subarachnoid hemorrhage inflammation repair

doi: 10.1186/s12951-025-03305-1

Figure Lengend Snippet: Schematic illustration of the preparation process for CAQK-incorporated and GsMTx4-loaded engineered endoplasmic reticulum vesicles (CAQKERM@GsMTx4) and th targeted drug delivery for subarachnoid hemorrhage (SAH)

Article Snippet: The endoplasmic reticulum membrane (ER membrane, EM), being the largest component of the intracellular membrane system, has not yet been fully explored or developed for use in biomimetic drug delivery systems.

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Engineered endoplasmic reticulum-targeting nanodrugs with Piezo1 inhibition and promotion of cell uptake for subarachnoid hemorrhage inflammation repair

doi: 10.1186/s12951-025-03305-1

Figure Lengend Snippet: Characterization of CAQKERM@GsMTx4. A Schematic illustration of CAQKERM@GsMTx4 preparation. B Flow cytometry analysis showing Flag-tagged Blank and CAQK expression (Flag) in transfected and non-transfected HEK293T cells. C Quantification of the mean fluorescence intensity in B . D Confocal microscope pictures of CAQK showing up on the cell membrane of HEK293T cells that had Flag-tagged CAQK-expressing plasmid (CAQK) and Blank plasmid (Blank) added to them E Confocal microscopy images showing colocalization of CAQK (Alexa Fluor™ 488-conjugated) with markers for the endoplasmic reticulum, mitochondria, and lysosomes in CAQK-expressing HEK293T cells. F Standard curve for detecting GsMTx4 using a spectrophotometer. G GsMTx4 loading efficiency in different vesicles ( n = 3). H Morphological characterization of EV’s, CAQK@EVs, CAQKERM@EVs, Blank@GsMTx4, CAQK@GsMTx4, and CAQKERM@GsMTx4 by TEM. Scale bar: 200 nm. I Size distribution analysis of EV’s, CAQK@EVs, CAQKERM@EVs, Blank@GsMTx4, CAQK@GsMTx4, and CAQKERM@GsMTx4 by nanoparticle tracking analysis. J SDS-PAGE and K Western blot analysis of membrane proteins

Article Snippet: The endoplasmic reticulum membrane (ER membrane, EM), being the largest component of the intracellular membrane system, has not yet been fully explored or developed for use in biomimetic drug delivery systems.

Techniques: Flow Cytometry, Expressing, Transfection, Fluorescence, Microscopy, Membrane, Plasmid Preparation, Confocal Microscopy, Spectrophotometry, SDS Page, Western Blot